Journal: Molecules

Article Title: Anticancer Effects of Ursi Fel Extract and Its Active Compound, Ursodeoxycholic Acid, in FRO Anaplastic Thyroid Cancer Cells

doi: 10.3390/molecules26175309

Figure Lengend Snippet: Effects of UF extract and UDCA on the expression of angiogenic proteins in human FRO anaplastic thyroid cancer cells. The cells were treated with UF or UDCA at indicated concentrations for 48 h. The expression of angiogenic proteins TGF-β ( a ), VEGF ( b ), N-cadherin (N-Cad) ( c ), and SIRT-1(d) in the cells was determined by western blotting. ( A ) β-Actin was used as an internal control. The expression of each protein was calculated relative to β-actin expression. Data are representative of three independent experiments. ( B ) * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. normal cells. N, untreated cells; UF25, 25 μg/mL UF extract treatment; UF50, 50 μg/mL UF extract treatment; UDCA50, 50 μM/mL UDCA treatment; UDCA100, 100 μM/mL UDCA treatment; D, 2 nM/mL docetaxel treatment.

Article Snippet: Anti-vascular endothelial growth factor (VEGF) Ab (sc-146) was procured from Novus Biologicals (E. Briarwood Avenue, CO, USA), and anti-sirtuin-1 (Sirt-1) Ab (bc-0921R) was obtained from Bioss Antibodies Inc. (Woburn, MA, USA).

Techniques: Expressing, Western Blot

Journal: The FASEB Journal

Article Title: An energy restriction‐based weight loss intervention is able to reverse the effects of obesity on the expression of liver tumor‐promoting genes

doi: 10.1096/fj.201901147rr

Figure Lengend Snippet: FIGURE 2 Plasma and tissue levels of oxidative stress biomarkers (A), plasma levels of inflammatory biomarkers (B), and hepatic expression of carcinogenesis-related genes and their protein levels in young and adult rats (C). Data are presented as the mean ± SEM (n = 7-10 animals/group, and n = 3 for expression levels). aStatistically significant differences compared with control lean counterparts (P < .05). bStatistically significant differences compared with the young group (P < .05). 8-OHdG, 8-hydroxy-2ʹ-deoxyguanosine; AOP, total antioxidative power; BIRC5, survivin; c-Myc, MYC proto-oncogene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GST-M2, glutathione-S-transferase; IL-6, interleukin-6; IL-10, interleukin-10; MCP-1, monocyte chemoattractant protein-1, PTEN, phosphatase and tensin homolog; SIRT1, sirtuin 1; TGFB, transforming growth factor-beta; TP53, tumor protein p53; TNF-α, tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor

Article Snippet: The primary monoclonal (mAb) and polyclonal (pAb) antibodies used were as follows: mouse anti-survivin mAb (dilution 1:500; catalog number sc-17779, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-MYC proto-oncogene (c-Myc) mAb (dilution 1:1,000; catalog number ab32072, Abcam Spain), rabbit anti-glutathioneS-transferase (GST-M2) pAb (dilution 1:1,000; catalog number ab175282, Abcam), mouse anti-sirtuin 1 (SIRT1) mAb (dilution 1:500; catalog number sc-74465, Santa Cruz Biotechnology), rabbit anti-transforming growth factor-beta (TGFB) pAb (dilution 1:1,000; catalog number 3711, Cell Signaling Technology, Danvers, MA, USA), goat anti-tumor protein p53 (TP53) pAb (dilution 1:1,000; catalog number AF1355, R&D Systems, Minneapolis, MN, USA), mouse anti-phosphatase and tensin homolog (PTEN) mAb (dilution 1:500; catalog number sc-7974, Santa Cruz Biotechnology), and anti-GAPDH mAb (dilution 1:2,000; catalog number AM4300, Life Technologies Ltd, Northumberland, UK).

Techniques: Clinical Proteomics, Expressing, Control

Journal: The FASEB Journal

Article Title: An energy restriction‐based weight loss intervention is able to reverse the effects of obesity on the expression of liver tumor‐promoting genes

doi: 10.1096/fj.201901147rr

Figure Lengend Snippet: FIGURE 3 Association between oxidative stress and transcript levels of carcinogenesis-related genes in adults rats. n = 10 animals/ group. BIRC5, survivin; SIRT1, sirtuin 1; TP53, tumor protein p53

Article Snippet: The primary monoclonal (mAb) and polyclonal (pAb) antibodies used were as follows: mouse anti-survivin mAb (dilution 1:500; catalog number sc-17779, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-MYC proto-oncogene (c-Myc) mAb (dilution 1:1,000; catalog number ab32072, Abcam Spain), rabbit anti-glutathioneS-transferase (GST-M2) pAb (dilution 1:1,000; catalog number ab175282, Abcam), mouse anti-sirtuin 1 (SIRT1) mAb (dilution 1:500; catalog number sc-74465, Santa Cruz Biotechnology), rabbit anti-transforming growth factor-beta (TGFB) pAb (dilution 1:1,000; catalog number 3711, Cell Signaling Technology, Danvers, MA, USA), goat anti-tumor protein p53 (TP53) pAb (dilution 1:1,000; catalog number AF1355, R&D Systems, Minneapolis, MN, USA), mouse anti-phosphatase and tensin homolog (PTEN) mAb (dilution 1:500; catalog number sc-7974, Santa Cruz Biotechnology), and anti-GAPDH mAb (dilution 1:2,000; catalog number AM4300, Life Technologies Ltd, Northumberland, UK).

Techniques:

Journal: Immunity, inflammation and disease

Article Title: Progranulin deficiency suppresses allergic asthma and enhances efferocytosis via PPAR-γ/MFG-E8 regulation in macrophages.

doi: 10.1002/iid3.779

Figure Lengend Snippet: FIGURE 6 Progranulin deficiency promoted the expression of milk fat globule‐epidermal growth factor 8 by upregulating peroxisome proliferators activated receptors‐γ in lung of asthmatic mice. (A) Protein levels of PPAR‐γ, MFG‐E8 and SIRT1 in mice lungs were analyzed by Western blot. The results of densitometric analysis were showed as means ± SD (n = 3). *p < .05, ***p < .001, ****p < .0001, unpaired t test. (B) Protocol of establishing OVA‐induced allergic airway diseases model. (C) The total number of leukocytes in BALF. Results were shown as means ± SD. (WT PBS, n = 5, WT OVA, n = 4, PGRN KO PBS, n = 3, PGRN KO OVA, n = 4, PGRN KO OVA + GW9662, n = 4) **p < .01, Welch's t‐test. (D) Protein levels of MFG‐E8 in mice lungs was analyzed by Western blot. (n = 2 or 3). The study was repeated for three times. Alum, aluminum; OVA, ovalbumin; PGRN, Progranulin; SD, standard deviation; WT, wild type.

Article Snippet: Antibodies used in our experiments were as follows: Anti‐cleaved caspase 3 (Cat. No 9664, clone 5A1E) and anti‐sirtuin 1 (SIRT1) (Cat. No. 3931 clone D60E1) were obtained from Cell Signaling Technology; anti‐MFG‐E8 (Cat. No. 377356, clone H‐3) was obtained from Santa Cruz; anti‐PPAR‐γ (Cat. No. ET1702‐57 clone JF101‐4) was obtained from HuaAn Biotechnology; anti‐PGRN (Cat. No. NBP1‐32076, polyclonal) was obtained from Novus Biologicals; anti‐GAPDH (Cat. No. 10494‐1‐AP polyclonal) was obtained from Proteintech and anti‐PE‐F4/ 80 Cat. No. 123109, clone BM8) was obtained from BioLegend.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Immunity, inflammation and disease

Article Title: Progranulin deficiency suppresses allergic asthma and enhances efferocytosis via PPAR-γ/MFG-E8 regulation in macrophages.

doi: 10.1002/iid3.779

Figure Lengend Snippet: FIGURE 5 Progranulin deficiency led to increased Sirtuin1 expression through peroxisome proliferators activated receptors‐γ and promoted interleukin‐10 production in efferocytes. (A) Western blot analysis of SIRT1 in PMs. The results of densitometric analysis were showed as means ± SD (n = 3). *p < .05, unpaired t test. (B) PMs were treated with DMSO or PPAR‐γ inhibitor GW9662 and assayed for SIRT1 protein after 24 h. The results of densitometric analysis were showed as means ± SD (n = 3). *p < .05, ***p < .001, ****p < .0001, unpaired t test. (C) PMs were cocultured with apoptotic cells for 12 h and Il10 levels were detected by q‐PCR. The relative mRNA expression was showed as means ± SD (n = 3). **p < .01, ***p < .001, unpaired t test. The study was repeated for three times. GW, GW9662; PM, peritoneal macrophage; PPAR‐γ, peroxisome proliferator‐activated receptor gamma; SD, standard deviation; SIRT1, sirtuin 1.

Article Snippet: Antibodies used in our experiments were as follows: Anti‐cleaved caspase 3 (Cat. No 9664, clone 5A1E) and anti‐sirtuin 1 (SIRT1) (Cat. No. 3931 clone D60E1) were obtained from Cell Signaling Technology; anti‐MFG‐E8 (Cat. No. 377356, clone H‐3) was obtained from Santa Cruz; anti‐PPAR‐γ (Cat. No. ET1702‐57 clone JF101‐4) was obtained from HuaAn Biotechnology; anti‐PGRN (Cat. No. NBP1‐32076, polyclonal) was obtained from Novus Biologicals; anti‐GAPDH (Cat. No. 10494‐1‐AP polyclonal) was obtained from Proteintech and anti‐PE‐F4/ 80 Cat. No. 123109, clone BM8) was obtained from BioLegend.

Techniques: Expressing, Western Blot, Standard Deviation